Welcome to NEBNext IS qPCR Absolute Quantification Tool

This tool analyzes qPCR amplification data and calculates absolute concentrations of libraries.

Workflow:

  1. Upload your Amplification Results CSV file
  2. Upload your Quantification Summary CSV file
  3. Set analysis parameters and target amplicon sizes
  4. Click 'Process Data'
  5. Go to 'Sample Management' to rename samples, exclude samples, and select highlighted samples
  6. Click 'Apply Changes' in Sample Management, then review plots and tables

Target Amplicon Sizes:

All target amplicon sizes are managed in a single field: one TARGET=SIZE per line.

IGH=700
BCR=635
TCR=660
MYC=450
BRAF=520
Note: Targets without a configured amplicon size are reported without size correction (factor = 1).

Manage Samples

  • New: edit to rename the sample
  • Exclude: check to remove from all analysis
  • Highlight: check to show in Highlighted Samples plot
Instructions:
1. Edit the 'New' column to rename samples
2. Check 'Exclude' to remove samples from analysis
3. Check 'Highlight' to include samples in highlighted plot
4. Click 'Apply Changes' below when done

Target Amplicon Size Configuration


Log-Linear Plot

Linear Plot (All Samples)

Linear Plot (Highlighted Samples)